Human adenovirus type 5 expressing FMDV serotype O (strain R2/75) capsid proteins and its immunogenicity in guinea pigs: Expression of FMD-VLPs  in Adenoviral expression system and evaluating its immunogenicity

Ramesh Kumar; B. P. Sreenivasa; Madhusudan Hosamani; Suresh H. Basagoudanavar; Mouttou Vivek Srinivas

doi:10.70964/avr.5

Authors

Ramesh Kumar FMD Vaccine Research Laboratory, ICAR- Indian Veterinary Research Institute, Hebbal, Bangalore, Karnataka - 560024 https://orcid.org/0000-0002-2944-7450
B. P. Sreenivasa FMD Vaccine Research Laboratory, ICAR- Indian Veterinary Research Institute, Hebbal, Bangalore, Karnataka - 560024, India https://orcid.org/0000-0002-6681-3288
Madhusudan Hosamani FMD Vaccine Research Laboratory, ICAR- Indian Veterinary Research Institute, Hebbal, Bangalore, Karnataka - 560024, India https://orcid.org/0000-0002-7346-7627
Suresh H. Basagoudanavar FMD Vaccine Research Laboratory, ICAR- Indian Veterinary Research Institute, Hebbal, Bangalore, Karnataka - 560024, India https://orcid.org/0000-0001-7714-3120
Mouttou Vivek Srinivas Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, India https://orcid.org/0000-0002-3931-3069

DOI:

https://doi.org/10.70964/avr.5

Keywords:

Foot-and-mouth disease, FMD VLP vaccine, New generation vaccine, Guinea pigs, Electron Microscopy, Adenoviral expression system, Virus like Particles

Abstract

Foot-and-mouth disease (FMD) is a highly contagious illness that significantly impacts cloven-hoofed animals and is of transboundary importance. The inactivated FMD vaccine is commonly used in countries like India to control the disease. Regular vaccination has helped to eradicate FMD in some countries and there are several concerns associated with the production and use of inactivated vaccines for FMD, which includes high costs associated with bio-containment facilities, potential risk of the virus escaping into the environment, and the vaccine's sensitivity to temperature changes. Therefore, the present study was aimed to develop a new generation FMD VLP vaccine using a Human adenovirus type-5 based expression system. In our study, the recombinant adeno-FMD virus showed efficient expression of the FMDV capsid proteins, which self-assembled into VLPs was observed by electron microscopy. The adeno-FMD vaccines protected fewer animals than conventional inactivated virus vaccines after homologous challenge. Recombinant adenovirus containing mutant 3C^prowas found superior to its wild-type counterpart in terms of protection. As no vaccine in the present study has given 100% protection, additional dose-response studies may provide information regarding the protective dose in guinea pigs. The data generated in the study suggest that the adeno-FMD vaccines may be an ideal alternative to the conventional inactivated virus vaccine.

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Human adenovirus type 5 expressing FMDV serotype O (strain R2/75) capsid proteins and its immunogenicity in guinea pigs

Expression of FMD-VLPs in Adenoviral expression system and evaluating its immunogenicity

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